The Genomics Facility houses a group of shared instruments used to analyze DNA, RNA, protein, and radio-labeled substances. All instruments are user-operated, but all users must first receive instruction on each instrument from the facility manager.
Role: Facility Manager
2720 Thermo Cycler is a small, 96 well platform model. It is a basic PCR instrument and new users need only minimal instruction to operate the instrument.
7500 Real-Time PCR System is an integrated and versatile platform for the detection and quantification of nucleic acid sequences. Real-Time PCR combines thermal cycling, fluorescence detection, and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube homogeneous reaction. Its five-color detection provides the flexibility to perform a variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays that utilize internal positive controls.
This instrument is housed in a darkroom on the 9th floor, Hunter North, behind the elevators. The Processor is designed to process medical x-ray sheet film.
For measuring fluorescence in liquid samples.
For measuring protein and DNA concentrations in liquid samples (measured in quartz cuvettes). Single sample measurements.
NanoDrop 1000 Spectrophotometer is a full spectrum (220nm-750nm) spectrophotometer which utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. It is able to measure 1 l samples with high accuracy and reproducibility.
Liquid scintillation counter with moving rack, designed to measure low-energy beta-emissions (e.g. 32P).
Provide training for operation of instrument and maintenance.