eagle-i Hunter College, CUNYHunter College, CUNY
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Genomic Facility

Summary:

The Genomics Facility houses a group of shared instruments used to analyze DNA, RNA, protein, and radio-labeled substances. All instruments are user-operated, but all users must first receive instruction on each instrument from the facility manager.

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    Instruments

    • Applied BioSystems 2720 Thermo Cycler ( PCR instrument )

      2720 Thermo Cycler is a small, 96 well platform model. It is a basic PCR instrument and new users need only minimal instruction to operate the instrument.

    • Applied BioSystems 7500 Real-Time PCR System ( Real-time PCR machine )

      7500 Real-Time PCR System is an integrated and versatile platform for the detection and quantification of nucleic acid sequences. Real-Time PCR combines thermal cycling, fluorescence detection, and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube homogeneous reaction. Its five-color detection provides the flexibility to perform a variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays that utilize internal positive controls.

    • Konica Minolta SRX-101A X-ray film Developer ( X-ray film processor )

      This instrument is housed in a darkroom on the 9th floor, Hunter North, behind the elevators. The Processor is designed to process medical x-ray sheet film.

    • Perkin-Elmer 650-10S Fluorescence Spectrophotometer ( Fluorescence spectrometer )

      For measuring fluorescence in liquid samples.

    • Perkin-Elmer UV/Visible Spectrophotometer Lambda 40 ( Spectrophotometer )

      For measuring protein and DNA concentrations in liquid samples (measured in quartz cuvettes). Single sample measurements.

    • Thermo Scientific NanoDrop 1000 Spectrophotometer ( Spectrophotometer )

      NanoDrop 1000 Spectrophotometer is a full spectrum (220nm-750nm) spectrophotometer which utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. It is able to measure 1 l samples with high accuracy and reproducibility.

    • Wallac 1409 Liquid Scintillation Counter ( Scintillation counter )

      Liquid scintillation counter with moving rack, designed to measure low-energy beta-emissions (e.g. 32P).

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    Last updated: 2015-06-23T12:02:36.338-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016