The Bio-Imaging facility currently provides optical and video microscopy services. The facility is equipped with Nikon TIRF-SIM super-resolution microscope, a Leica TCS SP2 confocal microscope, and a Perkin Elmer UltraVIEW ERS Spinning Disk system. In addition to confocal microscopy the facility is also equipped for bright field, phase contrast, modulation contrast and epi fluorescence microscopy. The facility has three separate wide field image analysis stations: a Nikon Optiphot upright microscope equipped with a color video system, a Nikon Eclipse TE200 inverted microscope with calcium ratio imaging capability, and a Nikon Eclipse Ti inverted system for ultra-fast imaging. The facility has recently expanded with the acquisition of a JEOL JEM-100C/CX Transmission Electron Microscope. The facility is also equipped with a Typhoon 9410 scanner for the quantitation and localization of sub cellular fluorescent and radioactive molecules, and an odyssey system for west-blotting imaging. In addition, Imaris, Volocity and Nikon Elements microscopy software are available for users.
Role: Facility Assistant
The calcium ratio imaging system consists of: a Nikon Eclipse TE 200 inverted epifluorescence microscope, sutter lambda 10-2 optical filter system, and Molecular Devices imaging software Metafluor. The system also is equipped with a Narishige micromanipulator system
"The Compix Imaging system consists of a Nikon Optiphot 2 upright microscope, and Nikon DXM 1200F high resolution (3,840 x 3,072 pixels) color video camera. The microscope is equipped for fluorescence, and has phase contrast and Nomarski optics. The system is interfaced to a 1.4Ghz PC which utilizes Compix Simple PCI image analysis software. The system also has Adobe Photoshop installed for image acquisition and manipulation."
"The Molecular Devices SpectraMax Gemini EM microplate spectrofluorometer features top and bottom reading optics, dual monochromators, wavelength scanning, well scanning, auto PMT gain and is driven by Softmax Pro software on a Windows-based controller."
"The GloMax®-96 Microplate Luminometer is a state-of-the-art microplate luminometer with a high sensitivity and broad dynamic range that is necessary for chemiluminescent and bioluminescent applications. The GloMax uses an advanced photon-counting photomultiplier tube (PMT), with a dynamic range of greater than nine logs. This range covers virtually all chemiluminescent and bioluminescent assays, eliminating the need to dilute samples or manage detector-driven gain changes."
"JEOL JEM-100CX II transmission electron microscope is an advanced high performance electron microscope with stable and excellent imaging capabilities at low to high magnifications (90X to 800, 000X). A 10M-pixel HAMAMASTU C4742-95 digital camera is integrated to the system for high-resolution image acquisition."
The Leica CM 3050S Cryostat features motorized sectioning and programmable defrost cycles. The cryostat can cut sections in the range 0.5 to 300 mm. The max specimen size is 55 X 70 mm, and can cool samples down to - 50°C.
"The TCS SP2 Laser Scanning Spectral Confocal Microscope can do measurements of transmitted light, fluorescence and laser scanning fluorescence imaging. It has a z-stage with rotation capabilities, with z-resolution of 40 nm. The system has high quality X10, X40, X63 and X100 objectives, and 6 excitation laser lines at wavelenghts of 458nm - 638 nm."
The Nikon TIRF SIM microscope allows the users to do both Total Internal Reflection Microscopy and SIM super resolution microscopy. The acquisition software is Nikon elements.
"The Odyssey replaces the tradition methods of analysing western blots, chemiluminescence and fluorescence detection, with near infrared (NIR) fluorescence detection. This allows you to quantitate proteins over a much wider linear dynamic range than chemiluminescence can. This feature can enable strong and weak bands on the same blot to be acurately quantified with out the need for multiple exposures. In addition the Odyssey is uniquely equipped with two infrared channels 700 nm and 800 nm, and can thus probe two different targets in the same experiment."
"The Ultraview is a spinning disk confocal microscope equipped with six laser lines, which allow visualization of GFP, RFP, CY5, DAPI, CFP and YFP, etc. The UltraVIEW ERS system allows for high-speed, multiple-probe, time-lapse experiments; fully automated image capture; time-lapse morphometric measurements; fast sequential image capture of multiple probes; and fast z-stack acquisition. UltraVIEW ERS Imaging Suite software and Volocity are used for fast image display, image acquisition and comprehensive image analysis. The system is supported by a Nikon Eclipse TE2000 inverted fluorescence microscope with environmental incubator which is ideal for live cell applications."
"PowerWave HT is a multi-channel reader for maximum speed in both 96- and 384-well plate formats. The PowerWave HT provides flexibility for multiple applications, including endpoint, kinetic and spectral scanning mode. Powerful Gen5™ PC-based software is used for system control and data analysis."
"Typhoon™ is a highly sensitive variable-mode gel imager. The Typhoon 9410 unites the ability to detect an extensive variety of fluors, with proven storage phosphor autoradiography technology and direct imaging of chemiluminescence. The typhoon can also be used to analyze micro-arrays."
Image data analysis
Training : life science instruments, including microscopes, plate readers, gel imagers.
"Now a new service is ready for our remote users to get remote access to our advanced confocal microscopes for their own imaging purpose.
Our service includes:
(i) Leica SP2 confocal microscope: it is ideal for regular 2D & 3D scanning for fixed slide samples. Please check the following link for Leica SP2 system: Leica SP2 operational guide;
(ii) Perkin Elmer spinning disk microscope: besides the regular 2D & 3D fixed slide scanning, it is also equipped with an environment chamber for live cell imaging. Also, this type of microscope system has fast scanning speed, it is ideal for cellular dynamic studies. Please check the following link for Perkin Elmer spinning disk microscope system: Spinning disk operational guide;
(iii) Microscope remote control: Webex is used to setup the remote desktop sharing for microscope control. Please check the following link for Webex-based remote control operations: Webex remote control guide;
(iv) PVX video conferencing for real-time consultation: during imaging experiment, PVX video conferencing system is used for real-time conversations between microscope operator and remote users to solve on-site experimental issues. Please check the following link for PVX real-time conferencing: PVX video conferencing operational guide.
(v) Cell staining protocol: a simple cell staining protocol is posted here as an example: Sample staining protocol.
For scheduling the above remote instrumentation service, please check the following guidelines:
(1) Contact us by email for scheduling a remote microscopic imaging experiment, including: a short description of your experiment, time schedule, sample type, etc.
Zhong Wang : email@example.com
Dr. Lloyd Williams: Williams@genectr.hunter.cuny.edu
(2) Ship the sample slide or living samples with proper package.
(3) A confirmation email will be sent before the experiment date. A webex meeting link will be included in this email for remote connection.
"Gen5™ Data Analysis Software incorporates over 30 years of experience and user feedback into outstanding microplate reader software. Gen5 is a unique combination of power and ease-of-use that drives productivity and saves time. Use Gen5 to control BioTek’s readers and export data, or as a fully integrated processing tool."
ImageQuant™ TL is the most automatic and easy-to-use general image analysis software. It offers four separate functional areas within one product:
1-D electrophoresis gels.
Dot blots, microplates, and basic arrays.
Colony counting and measurement of 2-D spots and other image features.
Toolbox to analyze gels with user-defined objects.
"Imaris is Bitplane’s core scientific software module that delivers all the necessary functionality for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size. Conveniently load, process and visualize data and images acquired from almost any confocal and wide field microscope to gain new and groundbreaking insight from your image data."
"MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years."
NIS Elements provides cutting edges tools for image manipulation, image analysis and data management.
"SoftMax® Pro Data Acquisition & Analysis Software serves as the user interface to the popular EMax®, VMax™, Gemini™, FilterMax™ and SpectraMax® Microplate Readers. Users are able to customize reader settings through the software to take advantage of unique reader technologies that improve the quality of assay data as well as do complex data analysis. Over 140 built in protocols along with protocol sharing community reduce the set up time for common applications."
Volocity® 3D Image Analysis Software is a solution that provides all the tools you need to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems and is fully integrated for a seamless user experience